75N95020Q00279 – Sources Sought – In-Vivo Fluorescent Microscope Calcium-Imaging Devise

Aug 28, 2020 | Sources Sought


This is a Small Business Sources Sought (SBSS) notice. This is NOT a solicitation for proposals, proposal abstracts, or quotations. The purpose of this notice is to obtain information regarding: (1) the availability and capability of qualified small business sources; (2) whether they are small businesses; HUBZone small businesses; service disabled, veteran-owned small businesses; 8(a) small businesses; veteran-owned small businesses; woman-owned small businesses; or small disadvantaged businesses; and (3) their size classification relative to the North American Industry Classification System (NAICS) code for the proposed acquisition. Your responses to the information requested will assist the Government in determining the appropriate acquisition method, including whether a set aside is possible. An organization that is not considered a small business under the applicable NAICS code should not submit a response to this notice.

This notice is issued to help determine the availability and type of qualified Small Business companies technically capable of meeting this potential government requirement and to determine the method of acquisition. It is not to be construed as a commitment by the Government to issue a solicitation or ultimately award a contract. Responses will not be considered as proposals or quotes. No award will be made as a result of this notice. The Government will NOT be responsible for any costs incurred by the respondents to this notice. The notice is strictly for research and information purposes only.

The information requested will assist the Government in determining the appropriate acquisition method including small business socioeconomic set aside possibilities and to determine the availability of qualified Small Business companies technically capable of meeting the Government’s potential requirement. All Small Business companies with the capability and availability to perform the requirement under the applicable NAICS code are invited to submit a response to this notice.


The intended procurement is classified under NAICS code 333314 – Optical Instrument and Lens Manufacturing

Manufacturing with a Size Standard of 500 employees.



The Neurocircuitry of Motivation Section aims to understand neural circuits that mediate compulsive behaviors such as drug-taking and drug-seeking behaviors.  We use rodent models to achieve this goal.  Compulsive behaviors such as drug-seeking are generated by the interactions of many types of neurons in the brain.  Histologically, researchers used microelectrodes to detect the activity of single neurons.  However, this method does not readily allow researchers to identify the types of neurons being recorded.  The fluorescence microscope procedure is a state-of-art technology, which allows researchers to record unit activity of neurons in a cell-type- and region-specific manner by expressing fluorophores that respond to calcium levels with viral technology.  The fluorescence microscope calcium imaging technology helps researchers to understanding how selective populations of neurons are activated during compulsive behavior in rodents.


The purpose is to acquire a fluorescence microscope system that allows us to detect fluorescence signals indicating cellular calcium levels in rodents. This device enables us to observe how specific types of cell populations get activated during the mouse perform compulsive behavior.

Project Requirements

In-vivo fluorescence microscope calcium imaging devise – Twist-on efocus Fluorescence Microscope System

Salient Characteristics

The system must be able to detect fluorescent signals emitted by GCaMP7f expressed on the surface of individual neurons and record such signals at a millisecond resolution for a few hours at a time. The system must include both hardware and software to accomplish this task. The microscope component must be small and light in such a way that a mouse can wear it on its head and move about a chamber freely for a few hours at a time.


Interested parties are expected to review this notice to familiarize itself with the requirements of this product or service. Failure to do so will be at your firm’s own risk.

Respondents must provide, as part of their response: (a) product, catalog, model, and/or part number(s); (b) product description; (c) best estimated delivery date after receipt of order; (d) all relevant information and documentation that the item(s) offered meets the salient physical, functional, or performance characteristics as specified in this announcement. Respondents that believe that they are authorized resellers of the equipment described in this announcement may provide, in addition to the aforementioned information, as part of their response: quantity; estimated price or cost; shipping, handling, and/or installation charges.

The respondent must also provide their DUNS number, organization name, address, point of contact, and size and type of business (e.g., 8(a), HubZone, etc.) pursuant to the applicable NAICS code and any other information that may be helpful in developing or finalizing the acquisition requirements.

One (1) copy of the response is required and must be in Microsoft Word or Adobe PDF format using 11point or 12point font, 8-1/2″ x 11″ paper size, with 1″ top, bottom, left and right margins, and with single or double spacing. The response is limited to ten (10) page limit. The 10-page limit does not include the cover page, executive summary, or references, if requested.

The information submitted must be in an outline format that addresses each of the elements of the product requirement. A cover page and an executive summary may be included but is not required.

The response must include the respondents’ technical and administrative points of contact, including names, titles, addresses, telephone and fax numbers, and email addresses.

Respondents must reference announcement number 75N95020Q00279 on all correspondence related to this Small Business Sources Sought notice. The response must be submitted electronically to alexander.beraud@nih.gov and kgoodlin@nida.nih.gov and be received prior to the closing date specified in this announcement.

The response must be received on or before September 4, 2020, 11:00 am, Eastern Time.


Disclaimer and Important Notes. This notice does not obligate the Government to award a contract or otherwise pay for the information provided in response. The Government reserves the right to use information provided by respondents for any purpose deemed necessary and legally appropriate. Any organization responding to this notice should ensure that its response is complete and sufficiently detailed to allow the Government to determine the organization’s qualifications to perform the work. Respondents are advised that the Government is under no obligation to acknowledge receipt of the information received or provide feedback to respondents with respect to any information submitted. After a review of the responses received, a pre-solicitation synopsis and solicitation may be published in beta.SAM. However, responses to this notice will not be considered adequate responses to a solicitation.

Confidentiality. No proprietary, classified, confidential, or sensitive information should be included in your response. The Government reserves the right to use any nonproprietary technical information in any resultant solicitation(s).

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