12405B20Q0092 – Sources Sought – Genome Assembly

Jul 31, 2020 | Sources Sought

The United States Department of Agriculture (USDA), Agricultural Research Service, is requesting information regarding the capability to provide fully assembled genomes of one hemipteran species (1,300 MBp) and one lepidopteran species (400 Mbp)genome assembly.

PURPOSE AND OBJECTIVES:

The primary objective of issuing the sources sought notice is to seek information from vendors with the capability of providing the following scientific service.

1. Preparing sequencing libraries using a proprietary technique (Chicago library method) to generate initial genomic contigs

2). Sequencing the libraries with Pacific Biosciences sequencing platform

3). Scaffolding the contigs with marker information using a proprietary assembly platform (HiRise) to obtain genomic scaffolds with high accuracy

4). Preparing proximity ligation libraries using proprietary nickase enzymes to yield markers across megabase length DNA molecules

5). Sequencing the proximity ligated libraries with Illumina platform

6). Scaffolding the genome to obtain near chromosome length scaffolds with high accuracy.

This is not a request for proposals, but instead market research to determine interested vendors prior to issuing a solicitation. Pricing information provided is for estimation purposes only. A determination by the Government on the method of competing this requirement has not been established. The Government will not award a contract based on this Sources Sought Notice or the information received, nor will it reimburse participants for the information they provide. If you are an interested vendor, please provide the following information: 1) Business name and point of contact; 2) DUNS number and CAGE code; 3) business size; and 4) a brief statement of Capability (no more than three pages) which demonstrates the ability to meet the specialized work requirements above. Include any past performance information on contracts of similar size and scope. Electronic responses are preferred. Information provided will not be used as a part of any subsequent solicitation requirement. Interested sources who submit data are responsible for appropriately marking information if it is proprietary in nature. A determination by the Government not to compete this proposed contract action based on this notice is solely within the discretion of the Government.

Responses are due no later than 2:00 p.m., Central Time, on Friday, August 07, 2020, to the attention of Jessica Pacheco, Contract Specialist, via e-mail to jessica.pacheco@usda.gov.

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